Czech Infrastructure for Integrative Structural Biology
CIISB - Czech Infrastructure for Integrative Structural Biology is formed by two Centers of Excellence for Structural Biology constructed within two projects: CEITEC – Central European Institute of Technology, Brno and BIOCEV - Biotechnology and Biomedicine Centre, Vestec, Prague-West. CEITEC and BIOCEV have been financed by the EU Structural Funds through the Operational Program Research and Development for Innovation, priority axis 1 – European Centers of Excellence, which is managed by the Ministry of Education, Youth and Sports of the Czech Republic. The Czech structural biology community is represented by the Czech Society for Structural Biology (CSSB), which is forming a national link to INSTRUCT. CIISB affiliation with INSTRUCT contributes to the development of human resources in research, attracts qualified national and international researchers, and enables efficient dissemination of knowledge and expertise within INSTRUCT, as well as the efficient use of the infrastructure.
The core facilities for structural biology in BIOCEV are organized under the Centre of molecular structure, run by the Institute of Biotechnology, Czech Academy of Sciences.
The Centre of Molecular Structure (CMS) encompasses several laboratories providing a complex approach to studies of three-dimensional structure, function and biophysical properties of biological molecules.
The BIOCEV building is located just outside of Prague. It can be easily reached by car or public transport. You will find detailed information on how to get to BIOCEV here.
Structural mass spectrometry (MS3D) offers various methodologies for characterisation of protein structure. The Institute of Biotechnology/Centre of Molecular Structure (IBT/CMS) at BIOCEV offers different labelling approaches including hydrogen/deuterium exchange, covalent labelling, chemical cross-linking and limited proteolysis. The facility is equipped with cutting-edge technologies including high-resolution mass spectrometer, HPLC system, H/D system and in-house software for data processing. The service provided includes data processing and reporting ready for publication. The platform also offers: identification and quantification of proteins, precise determination of protein molecular mass, and characterisation of various post translational modifications.
Techniques are available for the analysis of kinetic and thermodynamic parameters of biomolecular interactions and for the biophysical characterisation of the structure, function and stability of biological biological macromolecules like proteins, nucleic acids, lipids and their complexes.