Diffraction techniques core facility provides services for characterization of protein structure using single crystal X-ray diffraction and small-angle X ray scattering for analysis of liquid samples. Both methodes are backer by advanced approaches available in laboratory. The quality of high-intense X-ray beams is ensured by using of MetalJet sources (Excillum). The provided services include assistance with data processing and reporting ready for publication.
Preparation of biomolecule crystal samples for the measurement in the diffractometer. Handling of crystals, testing of cryo-conditions, cryo-cooling of crystalline samples.
Processing of X-ray diffraction data. Upon request, assistance with structure determination is also offered.
High performanece in-lab diffractometer for single crystal diffraction experiments. The instrument hosts MetalJet D2 X-ray source with Ga anode (λ=1.34Å) and large Photon III detector. The instrument is capable of both screening and data collection, including experimental phasing experiments.
In-situ diffraction in crysttalization plates
The versatile SAXSpoint 2.0 (Anton Paar) SAXS/WAXS device is equipped with the latest high brilliance C2+ liquid MetalJet source (liquid gallium alloy anode) and a Dectris EIGER 1M detector. Liquid samples are loaded automatically using the automated high performance sampler or manually in flow through measurement cells, including a high S/N silicon nitride measurement cell. Measurements are performed in the temperature range -10 °C to 120 °C. The state of the sample can be monitored online using in situ UV-Vis spectroscopy to identify radiation damage, and measure precise sample concentration and aggregation state.
Screening for X-ray diffraction of macromolecules
In-house data collection
In-situ evaluation of sensitive crystallization targets
Experimental phasing experiments – evaluation for MIR, SAD and MAD approaches
SAXS data measurement
Data processing and evaluation