Diffraction techniques core facility provides services for characterization of protein structure using single crystal X-ray diffraction and small-angle X ray scattering for analysis of liquid samples. The quality of high-intense X-ray beams is enabled by using of MetalJet sources (Excillum). The service provided includes data processing and reporting ready for publication.
Preparation of biomolecule crystal samples for the measurement in the diffractometer. Handling of crystals, testing of cryo-conditions, cryo-cooling of crystalline samples.
Processing of X-ray diffraction data. Upon request, assistance with structure determination is also offered.
D8 Venture diffractometer (Bruker) with a MetalJet D2 high-flux liquid Gallium X-ray source, Photon II detector and Kappa goniometer. This diffractometer is used for X-ray diffraction studies of biomolecular crystals (macromolecular crystallography). The device is also equipped with an ISX motorised stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallisation plates (plus limited diffraction data collection).
The versatile SAXSpoint 2.0 (Anton Paar) SAXS/WAXS device is equipped with the latest high brilliance C2+ liquid MetalJet source (liquid gallium alloy anode) and a Dectris EIGER 1M detector. Liquid samples are loaded automatically using the automated high performance sampler or manually in flow through measurement cells, including a high S/N silicon nitride measurement cell. Measurements are performed in the temperature range -10 °C to 120 °C. The state of the sample can be monitored online using in situ UV-Vis spectroscopy to identify radiation damage, and measure precise sample concentration and aggregation state.
Screening for X-ray diffraction of macromolecules
In-house data collection and processing
In-situ evaluation of sensitive crystallization targets, experimental phasing experiments – evaluation for MIR, SAD and MAD approaches
SAXS data measurement and evaluation
Synchrotron-based data collection and processing, structure solution