The Netherlands Centre for Electron Nanoscopy at Leiden University is the open access facility for cryo-TEM in the Netherlands.
The Netherlands Centre for Electron Nanoscopy (NeCEN) at Leiden University is part of the Instruct-NL national Instruct Centre in the Netherlands and provides access to cryo-transmission electron microscopy (cryo-TEM).
The NeCEN microscopes are based on the innovative Titan platform. NeCEN offers two main aspects of cryo-TEM. One of the microscopes is optimized for visualizing cellular structures in 3D with nanometer precision (tomography). The other microscope is equipped for analyzing single particles with even more detail. Cryo-transmission electron microscopy (cryo-TEM) allows scientists to observe proteins and organelles in a near native state.
In addition to data collection, NeCEN offers a wide range or cryo-EM related services, including sample preparation, sample screening, image processing and specialized customer tailored trainings and courses.
NeCEN is an open access facility and additionally accessible through Instruct and iNEXT-D. See the NeCEN website for detailed contact information.
NeCEN is an open access facility for high resolution cryo-electron microscopy of biological samples. Two state-of-the-art Titan Krios transmission electron microscopes allow efficient and automated high resolution data collection in its two most broadly used forms, single particle analysis (SPA) and cryo-electron tomography (cryo-ET). These imaging techniques allow visualisation of biomolecular structures, such as proteins, macromolecular complexes, bacteria, and cell organelles at subnanometer resolution in close-to-native conditions. The facility is equipped with a BSL2 lab and microscope rooms.