The Netherlands Centre for Electron Nanoscopy at Leiden University is the open access facility for cryo-TEM in the Netherlands.
The Netherlands Centre for Electron Nanoscopy (NeCEN) at Leiden University (external website) is part of the Instruct-NL national Instruct Centre in the Netherlands and provides access to cryo-transmission electron microscopy (cryo-TEM).
The NeCEN microscopes are based on the innovative Titan platform. NeCEN offers two main aspects of cryo-TEM. One of the microscopes is optimized for visualizing cellular structures in 3D with nanometer precision (tomography). The other microscope is equipped for analyzing single particles with even more detail. Cryo-transmission electron microscopy (cryo-TEM) allows scientists to observe proteins and organelles in a near native state.
In addition to data collection, NeCEN offers a wide range or cryo-EM related services, including sample preparation, sample screening, image processing and specialized customer tailored trainings and courses.
Important: to ensure a smooth application process, and to make sure users are aware of the potential additional costs involved, we strongly recommend any users to contact us before submitting an application.
NeCEN is an open access facility and additionally accessible through Instruct and iNEXT. See the NeCEN website for detailed contact information.
At NeCEN, there are two Thermo Scientific Titan Krios instruments available. The Titan Krios microscopes are dedicated to high-end data collection. Titan Krios 1 is a dedicated instrument for EFTEM (energy-filtered TEM) and cryo-tomography. This microscope has a S-FEG source and is equipped with a Gatan GIF+K2 DED (integrated into EPU allowing auto data collection) as well as a Thermo Scientific Falcon 2 DED camera and volta phase plate. It is an instrument for both tomography and automated single particle acquisition. Titan Krios 2 is a specialised instrument for single particle acquisition. This microscope has a X-FEG source, a Cs corrector, a Thermo Scientific Falcon 3 counting mode DED camera and volta phase plate.