The Netherlands Centre for Electron Nanoscopy at Leiden University is the open access facility for cryo-TEM in the Netherlands.
The Netherlands Centre for Electron Nanoscopy (NeCEN) at Leiden University (external website website) is part of the Instruct-NL national Instruct Centre in the Netherlands and provides access to cryo-transmission electron microscopy (cryo-TEM).
The NeCEN microscopes are based on the innovative Titan platform. NeCEN offers two main aspects of cryo-TEM. One of the microscopes is optimized for visualizing cellular structures in 3D with nanometer precision (tomography). The other microscope is equipped for analyzing single particles with even more detail. Cryo-transmission electron microscopy (cryo-TEM) allows scientists to observe proteins and organelles in a near native state.
In addition to data collection, NeCEN offers a wide range or cryo-EM related services, including sample preparation, sample screening, image processing and specialized customer tailored trainings and courses.
Important: to ensure a smooth application process, and to make sure users are aware of the potential additional costs involved, we strongly recommend any users to contact us before submitting an application.
NeCEN is an open access facility and additionally accessible through Instruct and iNEXT. See the NeCEN website for detailed contact information.
NeCEN is an open access facility for high resolution cryo-electron microscopy of biological samples. Two state-of-the-art Titan Krios transmission electron microscopes allow efficient and automated high resolution data collection in its two most broadly used forms, single particle analysis (SPA) and cryo-electron tomography (cryo-ET). These imaging techniques allow visualization of biomolecular structures, such as proteins, macromolecular complexes, bacteria, and cell organelles at subnanometer resolution in close-to-native conditions (no chemical fixation or staining required).