Instruct Centres - Instruct Centre - FR2


France 2 - The platforms of the Institut de Biologie Structurale managed by Integrated Structural Biology Grenoble (ISBG).

Integrated Structural Biology Grenoble (ISBG) & Institut de Biologie Structurale (IBS)

71 avenue des Martyrs

Grenoble

France

www.isbg.fr

Other Services/Technologies at Instruct Centre - FR2:

The RoBioMol platform, hosted by the Pneumococcus Group of the Institut de Biologie Structurale, and integrated in ISBG, offers high throughput molecular biology processes using automates.

Current services include:

  • gene cloning,
  • site-directed mutagenesis,
  • expression and purification test of proteins expressed in E. coli
  • plasmid preparation
  • screening of detergents for membrane protein solubilization and affinity purification
  • detergent exchange on solubilised membrane proteins.


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View All *Protein Production at Instruct

The Biophysics platform at ISBG & IBS, Grenoble offers one of the largest ensemble of state-of-the-art technic & methods for in vitro characterizing the intrinsic properties of biological macromolecules in solution, biomolecular interactions and their assemblages.



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View All *Macromolecular Interactions at Instruct

The Cellular Imaging platform grants the access to a confocal microscope (spinning disk S-M4D), a video-microscope (V-M4D), and a flow-cytometer (VYB). The instruments are optimized to study living cells. Details are available on the ISBG website.



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View All *Imaging at Instruct

3 electron microscopes:

Polara

High resolution single particle EM. K2 summit direct electron detector. Latitude S software for automated data collection

F20: cryoEM, cryo Tomo and negative staining. CETA camera

T12: negative staining, cellular EM Orius camera



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View All *Electron Microscopy at Instruct

ESPRIT: Screening of tens of thousands of constructs of a single gene to identify well-behaving soluble constructs.



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View All *Protein Production at Instruct

The Cell Free expression platform of IBS is devoted to large scale production (milligrams quantities) of soluble proteins, membrane proteins and RNAs for structural studies (X-ray, NMR, EM,...). The platform benefits from scientific input of the Membrane Transporters and Membrane and Immunity teams for expression and solubilisation of membrane proteins and from the Biomolecular NMR group for isotopic labelling of proteins and RNA. The Cell Free platform has implemented a quality management process for ISO 9001: 2015 certification, a key label and a warranty for all users of well-managed activities.



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View All *Protein Production at Instruct

The isotope labelling platform of the IBS is devoted to the large scale production of proteins uniformly or specifically enriched in stable isotopes (15N, 13C and 2H) for biomolecular NMR spectroscopy studies. The platform is offering access to innovative labelling schemes, developed locally on the platform by the IBS. The platform benefits from extensive scientific contacts with the IBS high field NMR Spectroscopy and Cell-Free platforms. Activities proposed by the platform: Users have access to the facility for large scale expression of labelled proteins in E.coli, under the supervision of a qualified platform engineer. Dedicated bench, optimised protocols and adequate isotopically labelled materials are available to users.



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View All *Protein Production at Instruct

The mass spectrometry (MS) platform provides the scientific community a service for protein analysis and characterization and offers the following analytical techniques : - Determination of exact mass, at high resolution, for intact proteins and peptides - Control of protein and peptide expression, modification, mutation and labeling - Analyses of protein limited proteolysis - Analysis of macromolecular complexes by native MS.



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View All *Mass Spectrometry at Instruct

Scientific Contacts
Darren Hart
Darren Hart

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Winfried Weissenhorn
Winfried Weissenhorn

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Admin Contacts
Darren Hart
Darren Hart

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Linda Ponnet
Linda Ponnet

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Technical Contacts
Caroline Mas
Caroline Mas

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Bernhard Brutscher
Bernhard Brutscher

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Philippe Mas
Philippe Mas

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Jerome BOISBOUVIER
Jerome BOISBOUVIER

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Lionel Imbert
Lionel Imbert

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Michel Thépaut
Michel Thépaut

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Vernet Thierry
Vernet Thierry

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Anne-Marie Villard
Anne-Marie Villard

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Luca Signor
Luca Signor

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Elisabetta Boeri Erba
Elisabetta Boeri Erba

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Françoise Lacroix
Françoise Lacroix

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Jean-Philippe KLEMAN
Jean-Philippe KLEMAN

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jean-baptiste reiser
jean-baptiste reiser

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Aline Le Roy
Aline Le Roy

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Guy Schoehn
Guy Schoehn

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Adrien Favier
Adrien Favier

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Florine Dupeux
Florine Dupeux

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Anne CHOUQUET
Anne CHOUQUET

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Isabel Ayala
Isabel Ayala

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