France 2 - The platforms of the Institut de Biologie Structurale managed by Integrated Structural Biology Grenoble (ISBG).
The RoBioMol platform, hosted by the Pneumococcus Group of the Institut de Biologie Structurale, and integrated in ISBG, offers high throughput molecular biology processes using automates.
Current services include:
The Biophysics platform at ISBG & IBS, Grenoble offers one of the largest ensemble of state-of-the-art technic & methods for in vitro characterizing the intrinsic properties of biological macromolecules in solution, biomolecular interactions and their assemblages.
The Cellular Imaging platform grants the access to a confocal microscope (spinning disk S-M4D), a video-microscope (V-M4D), and a flow-cytometer (VYB). The instruments are optimized to study living cells. Details are available on the ISBG website.
3 electron microscopes:
High resolution single particle EM. K2 summit direct electron detector. Latitude S software for automated data collection
F20: cryoEM, cryo Tomo and negative staining. CETA camera
T12: negative staining, cellular EM Orius camera
ESPRIT: Screening of tens of thousands of constructs of a single gene to identify well-behaving soluble constructs.
The Cell Free expression platform of IBS is devoted to large scale production (milligrams quantities) of soluble proteins, membrane proteins and RNAs for structural studies (X-ray, NMR, EM,...). The platform benefits from scientific input of the Membrane Transporters and Membrane and Immunity teams for expression and solubilisation of membrane proteins and from the Biomolecular NMR group for isotopic labelling of proteins and RNA. The Cell Free platform has implemented a quality management process for ISO 9001: 2015 certification, a key label and a warranty for all users of well-managed activities.
The isotope labelling platform of the IBS is devoted to the large scale production of proteins uniformly or specifically enriched in stable isotopes (15N, 13C and 2H) for biomolecular NMR spectroscopy studies. The platform is offering access to innovative labelling schemes, developed locally on the platform by the IBS. The platform benefits from extensive scientific contacts with the IBS high field NMR Spectroscopy and Cell-Free platforms. Activities proposed by the platform: Users have access to the facility for large scale expression of labelled proteins in E.coli, under the supervision of a qualified platform engineer. Dedicated bench, optimised protocols and adequate isotopically labelled materials are available to users.
The mass spectrometry (MS) platform provides the scientific community a service for protein analysis and characterization and offers the following analytical techniques : Determination of exact mass, at high resolution, for intact proteins and peptides Control of protein and peptide expression, modification, mutation and labeling Analyses of protein limited proteolysis Analysis of macromolecular complexes by native MS.