Strasbourg provides an integrated environment for structural studies of protein and complexes from sample preparation to structure determination.
The Strasbourg centre provides a cutting-edge technological environment for integrative structural biology approaches, from the molecular to the cellular level, with an emphasis on protein production linked with preparation of samples for EM and crystallography.
Protein engineering and production are essential tools for structural, biophysical and functional studies as well as for biotechnology and medical applications. The Strasbourg center offers a state-of-the-art infrastructure for the preparation of biomolecules and biomolecular complexes: recombinant expression in E. coli, insect or mammalian cells as well as biomass production.
Strasbourg provides core-technologies for structure determination by crystallography, cryo-EM and tomography. Strasbourg is a centre of expertise for crystallographic analysis of protein-protein and protein-nucleic acid complexes (nano and micro-automated screening and HTP crystal production, data collection and structure determination) and cryo-electron microscopy/tomography (Hight resolution cryo-EM, cryo electron tomography using up to date equipment (Polara and Titan microscopes and Falcon detectors).
The centre also offers access to:
The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis.
Biological Small-Angle X-Ray Scattering (Bio-SAXS) can be used to collect low resolution structural data, or for understanding the subunit arrangement of multi-subunit macromolecules including in the presence of a ligand. A Rigaku BioSAXS-1000 is attached to the right port of a Rigaku MicroMaxTM-007HF generator. The BioSAXS-1000 is fitted with Osmic optics and a 2D Kratky block for collimation. Data is collected on a Pilatus 100K photon counting detector (Dectris).
The SR GSD allows a range of localization super-resolution experiments (dSTORM, GSDIM, STORM, PALM), particle tracking, standard wide-field epifluorescence and TIRF microscopy, transmission microscopy with polarization contrast. Samples can be mounted either on standard slides or in 35mm diameter glass bottom petri dishes.
Liquid state NMR
NMR spectroscopy provides invaluable insights into the structural and dynamical features of biomolecular systems. We are taking advantage of NMR, combined with other structural biology methods, to investigate molecular properties that are important to achieve a given biological function.
Three spectrometers are installed in the lab: a 500 MHz with room temperature probes, a 600 MHz and a 700 MHz with cryo-probes to achieve highest sensitivity.
500 MHz spectrometer: For experiments that don't need best sensitivity and resolution, a 500 MHZ with two room temperature probes is available (a selective proton probe with carbon and nitrogen decoupling and Z gradients;a broadband probe that allows observation of 19F as well as 31P, 13C and 15N with 1H decoupling.
600 MHz spectrometer: For experiments that need high sensitivity and resolution, the 600 MHz spectrometer with cryogenic probe is available (selective proton CryProbeTM with carbon, nitrogen and deuterium decoupling and Z gradients).
700 MHz spectrometer: For experiments that need extreme sensitivity and resolution, the 700 MHZ spectrometer with a selective proton and carbon CryoProbeTM with nitrogen/carbon/deuterium decoupling and Z gradients.
The high throughput crystallisation platform offers vapour diffusion as well as crystallisation under oil screening and optimisation in sitting drops. Flexible methods have been developed to adapt each step to individual project requirements. At the crystallisation level, we have especially focused on developing techniques to optimise crystal growth.
Instruct Centre-FR1 offers state-of-the-art equipment and expertise to perform biophysical analysis and quality control on protein samples. Techniques include Analytical ultracentrifugation (AUC), Isothermal titration calorimetry (ITC) and Microscale Thermophoresis.
Sample preparation is not only a key issue for structural and molecular biology studies, but concerns life sciences development as a whole. Instruct centre France 1 offers state-of-the-art infrastructures for the expression and production of biomolecules and their complexes. The center proposes recombinant expression of protein and multi-protein complexes in prokaryotic and eukaryotic hosts as well as biomass production for isolation of poorly abundant proteins and endogenous complexes.
Liquid state NMR
NMR spectroscopy provides invaluable insights into the structural and dynamical features of biomolecular systems. We are taking advantage of NMR, combined with other structural biology methods, to investigate molecular properties that are important to achieve a given biological function.
Three spectrometers are installed in the lab: a 500 MHz with room temperature probes, a 600 MHz and a 700 MHz with cryo-probes to achieve highest sensitivity.
500 MHz spectrometer: For experiments that don't need best sensitivity and resolution, a 500 MHZ with two room temperature probes is available (a selective proton probe with carbon and nitrogen decoupling and Z gradients;a broadband probe that allows observation of 19F as well as 31P, 13C and 15N with 1H decoupling.
600 MHz spectrometer: For experiments that need high sensitivity and resolution, the 600 MHz spectrometer with cryogenic probe is available (selective proton CryProbeTM with carbon, nitrogen and deuterium decoupling and Z gradients).
700 MHz spectrometer: For experiments that need extreme sensitivity and resolution, the 700 MHZ spectrometer with a selective proton and carbon CryoProbeTM with nitrogen/carbon/deuterium decoupling and Z gradients.
