Service / Technology Instance

About Cell Imaging, Strasbourg, France

View All at Instruct

The SR GSD allows a range of localization super-resolution experiments (SMLM: dSTORM, GSDIM, STORM, PALM, PAINT), particle tracking, standard wide-field epifluorescence and TIRF microscopy, transmission microscopy. Samples can be mounted either on standard slides or in 35mm-diameter glass bottom petri dishes (preferred for liquid mounting media).

Instruments Available:

The SR GSD allows a range of localization super-resolution experiments (SMLM: dSTORM, GSDIM, STORM, PALM, PAINT), particle tracking, standard wide-field epifluorescence and TIRF microscopy, transmission microscopy. Samples can be mounted either on standard slides or in 35mm-diameter glass bottom petri dishes (preferred for liquid mounting media).

User Guide

The Leica SR GSD system is based on the Leica DMI6000 B inverted microscope that is equipped with a TIRF attachment, 160x/1.43 oil immersion and 10x/0.40 dry objectives. The maximal achievable resolution, depending on specimen, lies in range from 20 to 70 nm; the localization precision of individual fluorophores can be as high as a few nanometers.

Laser lines that can be used for excitation of fluorescence and photoconversion: 488 nm 300 mW, 532 nm 1000 mW, 642 nm 500 mW, 405 nm 50 mW. The images are captured by the Andor iXon3 897 EMCCD camera with the equivalent pixel size of 100 nm. The field of view with the 160x objective is 51x51 µm in conventional wide-field mode and 18x18 µm - 40x40 µm for super-resolution imaging.

The microscope is additionally equipped with:

  • The Optosplit II Dual Emission Image Splitter that allows for simultaneous multi-color imaging using appropriate fluorophores.
  • A cylindrical lens for astigmatism-based 3D imaging.
  • The MicAO 3D-SR adaptive optics system that permits detection and correction of optical aberrations in the sample as well as generation of astigmatism for 3D experiments.

The microscope is driven by the LAS AF software that allows for the full control of experiments and super-resolution image processing in real time. Other software available for users: SharpViSu, ClusterViSu, ImageJ.

The microscopy room is equipped with laboratory tools for sample preparation (e.g., immunofluorescence, mounting) and their storage at +4 °C/-20 °C.

Stock of various mounting media (Glucose Oxidase/Catalase, OxyFluor, Vectashield and common additives) as well as dye-conjugated secondary antibodies are available.

Instruct Centre

Instruct Centre FR1

IGBMC

1, rue Laurent Fries

Illkirch

Strasbourg

France

www.igbmc.fr

Cell Imaging, Strasbourg, France

Contacts:

Marie-Christine Poterszman
Marie-Christine Poterszman
CNRS
Login to contact
Leonid Andronov
Leonid Andronov
IGBMC-CERBM
Login to contact
Bruno Klaholz
Bruno Klaholz
IGBMC-CERBM
Login to contact