The NMR instruments available at the Bijvoet Centre are state-of-the-art Bruker spectrometers, suitable for all the standard pulse sequences. Tailored pulse sequences for structural determination of high molecular weight proteins and other dedicated applications are developed and available.
The Bijvoet Center at UU offers access and support for solution NMR studies of biomolecules at field strengths ranging from 600 to 950 MHz. The instruments are well-equipped for all standard structural and dynamical characterization of proteins or other biomolecules, with cryoprobes for enhanced sensitivity on three machines (600, 900, and 950 MHz). A dedicated Argon-laser setup is available on a 500 MHz spectrometer for light-sensitive experiments.
The Facility has extensive expertise in the study of protein structure, dynamics and interactions. Applications involving high molecular weight proteins or protein complexes, such as nucleosome-protein complexes (200-250 kDa) for instance, benefit from the available tailored pulse sequences, provided these samples are appropiately isotope-labelled. We can now also offer dedicated support for applications involving interactions of intrinsically disordered proteins.
For more information, please visit the website of the SONNMRLSF facility at the Bijvoet Center.