Czech Infrastructure for Integrative Structural Biology
CIISB - Czech Infrastructure for Integrative Structural Biology is formed by two Centers of Excellence for Structural Biology constructed within the projects CEITEC – Central European Institute of Technology, Brno and BIOCEV - Biotechnology and Biomedicine Centre, Vestec, Prague-West. CEITEC and BIOCEV have been financed from the EU Structural Funds through the Operational Program Research and Development for Innovation, priority axis 1 – European Centers of Excellence, which is managed by the Ministry of Education, Youth and Sports of the Czech Republic. The Czech structural biology community is represented by the Czech Society for Structural Biology (CSSB), which is forming a national link to INSTRUCT. CIISB affiliation with INSTRUCT contributes to the development of human resources in research, attracts qualified national and international researchers, and enables efficient dissemination of knowledge and expertise within INSTRUCT, as well as efficient use of the infrastructure.
CEITEC is a scientific centre of excellence in the fields of life sciences, advanced materials, and technologies, which aims to establish itself as a recognized centre for basic as well as applied research. A consortium of partners includes the most prominent universities and research institutes in Brno, Czech Republic. CEITEC Core Faciilties for Integrative Structural Biology are part of the Structural Biology Program comprising research groups focusing on molecular biology and biochemical and structural characterization of cellular processes, including RNA, studies of the ribosome, understanding the structure-function relationships of proteins in pathogenic bacteria, fungi, and viruses, and on the acquisition of knowledge necessary for the development of biosensors for nanomechanical detection of clinical markers using their in vivo/in situ detection in real time.
The new CEITEC building is located on Masaryk University Campus in the southwest part of Brno. The site can be easily reached by car, bus or train. More detailed information about how to get to CEITEC is available HERE.
Key equipment: High-end electron microscope for high-resolution cryoEM and cryo-tomography FEI Titan Krios (80 - 300 kV) equipped with an energy filter and a direct detector camera. Conventional cryoEM microscope FEI F20 (200 kV) equipped with a CCD camera and a dual beam FIB/SEM instrument (Versa3D) for micromachining of thin lamellas of vitrified cells usable for electron tomography.
The platform provides services leading to biophysical and structure characterization of biomolecules and to study (bio)molecular interactions. It is equipped with the instrumentation to set up crystallization conditions of biomolecules and their complexes, basic characterization of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation).
The platform provides instruments and services leading to the biophysical characterization of (bio)molecules, the study of biomolecular interactions (determination of affinity, kinetic and thermodynamic parameters) and the sample quality control using analytical ultracentrifugation (AUC), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), microscale thermophoresis (MST), dynamic light scattering (DLS), differential scanning calorimetry (DSC) and differential scanning fluorimetry (DSF). The platform is available to entire INSTRUCT-ERIC community.
Scanning probe microscopies for imaging of biological samples - tissue slices, cells, single biomolecules as nucleic acids and proteins. Studies of affinity interactions at the molecular level.
We provide comlete MS-assisted proteomics services including sample preparation, separation of protein/peptide mixtures, qualitative and quantitative characterisation of proteins and their modifications by mass spectrometry and data processing.
Core Facility of High Field NMR Spectroscopy provides access to NMR spectrometers in the range of proton frequencies from 500 MHz to 950 MHz. The equipment is suited mainly to the studies of structure, dynamics and interactions of biomolecules, i.e. proteins, nucleic acids and carbohydrates. However, the instrumentation is flexible enough to cover also various research needs in material science, organic and inorganic chemistry, biochemistry, biology and biophysics.
NMR (Nuclear Magnetic Resonance) spectroscopy is a key technology for
research in modern life sciences allowing detailed investigation of
biomolecular structure and dynamics at the atomic level, both in
solutions and in solid state. The successful application of NMR in
biology requires multidisciplinary approach combining knowledge in
biochemistry, molecular biology, quantum physics, electronics, data
analysis, and computational chemistry. The high-end instrumentation and
the team of experienced researchers ensures expert services, user
training, and the cost-effective use of resources. Benefits include access to state-of-the-art high-field
NMR instrumentation and support in processing, analysis and
interpretation of the experimental data. External user projects are selected by peer review on the basis of scientific merit, technical
suitability and feasibility. The Centre also offers training
enabling non-specialists to develop the necessary skills.
X-ray Diffraction and Bio-SAXS core facility is equipped with top-class instruments for diffraction experiments with single crystal samples focused on the determination of the 3-D structure of (macro) molecules down to atomic resolution and for small angle X-ray scattering (SAXS) experiments with isotropically scattering samples focused on determination of the shape and size of macromolecules or nanoparticles.
The range of applicable molecular mass for diffraction methods: from 100 up to 1000000, where the lower value covers molecules significant for nanotechnology, materials science or pharmacology and the upper limit covers biomacromolecules such as nucleic acids, proteins and their complexes.
Range of applicable particle sizes for SAXS: from 2 to 100 nm.