Service / Technology Instance

About Electron Microscopy, Brno, Czech Republic

The cryo-EM facility at CEITEC provides access and support to those needing to collect cryo-electron microscopy images for both single particle and electron tomography applications. In addition, the facility can provide support for sample preparation and experiment design.

 

 

Instruments Available:

High-end electron microscope for high-resolution cryoEM and cryo-tomography FEI Titan Krios (80 - 300 kV) equipped with an energy filter and a direct detector camera. Conventional cryoEM microscope FEI F20 (200 kV) equipped with a CCD camera and a dual beam FIB/SEM instrument (Versa3D) for micromachining of thin lamellas of vitrified cells usable for electron tomography. Thermo Scientific Talos Arctica – mid-range transmission electron microscope operating at 40 - 200 kV equipped with Schottky field emission gun (XFEG) providing highly coherent electron beam with ultimate stability. The microscope is equipped with Thermo Scientific Falcon3 EC direct electron detector and Ceta camera. Automated collection data for single particle analysis and cryo-electron tomography data can be performed with EPU or SerialEM software, respectively. The microscope is well suited for automated acquisition of high-quality cryoEM data, efficient screening of cryoEM samples, and 3D electron diffraction tomography

User Guide

Key equipment

High-end electron microscope for high-resolution cryoEM and cryo-tomography FEI Titan Krios (80 - 300 kV) equipped with an energy filter and a direct detector camera. Conventional cryoEM microscope FEI F20 (200 kV) equipped with a CCD camera and a dual beam FIB/SEM instrument (Versa3D) for micromachining of thin lamellas of vitrified cells usable for electron tomography.

Instruments available

  • FEI Titan Krios – the microscope operates at 300 kV and is equipped with an autoloader, FEI Falcon II direct detector, Gatan Quantum 964 energy filter and a post-GIF 4k CCD camera. The microscope is optimally suitable for high-resolution single particle cryo-electron microscopy and cellular cryo-electron tomography
  • FEI Tecnai F20 – the microscope operates at 120–200 kV and is equipped with an FEI Eagle 4k CCD camera, two side-entry cryo-holders (Gatan 626 and Gatan 914) and two room-temperature side-entry holders. The microscope is optimally equipped for optimization of cryoEM samples and automated collection of data for initial 3-D reconstructions by single particle analysis
  • FEI Versa3D – this small dual beam microscope (SEM/FIB) is equipped with a Quorum cryo-stage for insertion and processing of cryo-TEM grids (imaging by SEM and lamellae milling with FIB). The samples can be Pt-coated inside the microscope

Services provided

The cryo-EM core facility provides access and support to collect cryo-electron microscopy images for both single particle as well as for electron tomography applications. Furthermore, the facility provides support for sample preparation, namely plunge freezing of purified protein complexes as well as cryo-FIB lamella milling for thick biological objects such as cells. Additional support is given in experiment design, setting up data collection and data analysis (i.e. image processing). Interested non-specialists could receive training in using the electron microscopes or develop a collaboration with the CryoEM research group, particularly in these areas:

  • High-resolution structure determination of macromolecular complexes and assemblies
  • Structural studies of intracellular compartments and host-pathogen interactions
  • Time-resolved electron microscopy of transient macromolecular complexes

Instruct Centre

CEITEC

CEITEC

Kamenice 753/5

625 00

Brno

Czech Republic

www.ceitec.eu

Electron Microscopy, Brno, Czech Republic

Contacts:

Jiri Novacek
Jiri Novacek
CEITEC - Central European Institute of Technology
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Katerina Vagnerova
Katerina Vagnerova
CEITEC - Central European Institute of Technology
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Radka Matejickova
Radka Matejickova
CEITEC - Central European Institute of Technology
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