France 2 - The platforms of the Institut de Biologie Structurale managed by Integrated Structural Biology Grenoble (ISBG).
Instruct Centre France 2 in Grenoble provides technician-supported user access to some of the highest level structural biology instrumentation in France. Our services/technologies address areas of sample preparation, characterisation and structural determination. All platforms have ISO 9001 quality certification. Our platforms are located at the Institut de Biologie Structurale (IBS) with user access managed by the Integrated Structural Biology Grenoble (ISBG) service unit.
The IBS is on the EPN Science Campus, an international science hub that also hosts three major European institutes – EMBL, ESRF and ILL, each with major research and user access programs in structural biology.
The new IBS building is located on EPN Science campus in the northern part of Grenoble. The site can be easily reached by car, bus or train thanks to the nearby motorway connections and railway station. Visitors to the EPN science campus must be registered for a visit prior to arrival. Please fill in this form and give it to your contact more than 48h in advance. You will need to produce proof of identification (e.g. passport, ID card) to be able to enter. The site entrance is located 71 avenue des Martyrs.
The EM platform at IBS provides access to a full range of instruments (three in total) for national and European users via FRISBI and Instruct respectively. This includes classical quality control negative staining experiment (Tecnai 12 EM) prior to setting up cryo conditions (F20 EM equipped for cryo and tomography). Data acquisition can also be carried out on the F20 EM. Our third EM: the Glacios is equipped with a direct electron detector ((Falcon II). The Glacios is able to do single particle imaging and single axis tomo.
The Molecular Biology and Protein Expression Automated Platform, RoBioMol, is hosted by the Pneumococcus Group of the Institut de Biologie Structurale (IBS) and offers high throughput molecular biology processes using automates.
The Cellular Imaging platform allows the access to a confocal microscope (spinning disk S-M4D), a video-microscope (V-M4D), and a flow-cytometer (VYB). The instruments are optimised to study living cells. Details are available on the ISBG website.
Expression of Soluble Proteins by Random Incremental Truncation (ESPRIT) is a directed evolution-type process combining random deletion mutagenesis with high throughput solubility screening.
Lipidic cubic phase (LCP) crystallisation is an alternative to standard crystallisation for membrane proteins. It allows membrane proteins to crystallise in a close to native and stable environment, in a lipidic bilayer. G-protein-coupled receptors (GCPR) protein crystallisation demonstrated the power of this approach; Cherezov V et al. High Resolution Crystal Structure of an Engineered Human β2-Adrenergic G protein-Coupled Receptor. As LCP is described as infinite 3D periodic structure, membrane proteins (MP) can freely diffuse laterally along the membrane. Diffusion is one of the important pre-crystallisation characteristic of the MP embedded into lipidic bilayer, which could be checked by Fluorescence recovery After Photobleaching (FRAP) technique available at HTMPC platform.
The Biophysics platform at Integrated Structural Biology Grenoble (ISBG) and Institut de Biologie Structurale (IBS), Grenoble offers one of the largest ensemble of state-of-the-art technic & methods for in vitro characterising the intrinsic properties of biological macromolecules in solution, biomolecular interactions and their assemblages.
The mass spectrometry (MS) platform can be used for the analysis and characterisation of proteins and offers the following analytical techniques: determination of exact mass at high resolution for intact proteins and peptides, control of protein and peptide expression, modification, mutation and labelling , analyses of protein limited proteolysis, and analysis of macromolecular complexes by native MS.
The Cell Free expression platform of IBS is devoted to large scale production (milligrams quantities) of soluble proteins, membrane proteins and RNAs for structural studies (X-ray, NMR, EM,...). The platform benefits from scientific input of the Membrane Transporters and Membrane and Immunity teams for expression and solubilisation of membrane proteins and from the Biomolecular NMR group for isotopic labelling of proteins and RNA. The Cell Free platform has implemented a quality management process for ISO 9001: 2015 certification, a key label and a warranty for all users of well-managed activities.
The isotope labelling platform of the Institut de Biologie Structurale (IBS) is devoted to the large scale production of proteins uniformly or specifically enriched in stable isotopes (15N, 13C and 2H) for biomolecular NMR spectroscopy studies. The platform offers access to innovative labelling schemes, developed locally on the platform by the IBS. The platform benefits from extensive scientific contacts with the IBS high field NMR Spectroscopy and Cell-Free platforms. Activities proposed by the platform: Users have access to the facility for large scale expression of labelled proteins in E.coli, under the supervision of a qualified platform engineer. Dedicated bench, optimised protocols and adequate isotopically labelled materials are available to users.
Solid state nuclear magnetic resonance equipment available through Instruct at Grenoble include a 950 MHz and several 600 MHz instruments, equipped with state-of-the-art magic-angle spinning (MAS) capabilities, and triple-resonance probes for different rotor sizes and spinning speeds.
Researchers can gain access to 950, 850, 700, and several 600 MHz instruments, equipped with most recent Bruker electronics (Avance III HD) and cryogenically cooled probes for high-sensitivity solution-state NMR applications. Standard Bruker experiment libraries, as well as additional in-house libraries for optimised fast NMR data acquisition (e.g. SOFAST, BEST, and HADAMAC-type experiments) are available. Fast-mixing equipments required for real-time studies of kinetic processes such as protein folding are also available.