Service / Technology Instance

About X-ray Diffraction and Bio-SAXS, Vestec near Prague, Czech Republic

View All at Instruct

Diffraction techniques core facility provides services for characterization of protein structure using single crystal X-ray diffraction and small-angle X ray scattering for analysis of liquid samples. The quality of high-intense X-ray beams is enabled by using of MetalJet sources (Excillum). The service provided includes data processing and reporting ready for publication.

 

Instruments Available:

Preparation of biomolecule crystal samples for the measurement in the diffractometer. Handling of crystals, testing of cryo-conditions, cryo-cooling of crystalline samples.

Processing of X-ray diffraction data. Upon request, assistance with structure determination is also offered.

D8 Venture diffractometer (Bruker) with a MetalJet D2 high-flux liquid Gallium X-ray source, Photon II detector and Kappa goniometer. This diffractometer is used for X-ray diffraction studies of biomolecular crystals (macromolecular crystallography). The device is also equipped with an ISX motorised stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallisation plates (plus limited diffraction data collection).

The versatile SAXSpoint 2.0 (Anton Paar) SAXS/WAXS device is equipped with the latest high brilliance C2+ liquid MetalJet source (liquid gallium alloy anode) and a Dectris EIGER 1M detector. Liquid samples are loaded automatically using the automated high performance sampler or manually in flow through measurement cells, including a high S/N silicon nitride measurement cell. Measurements are performed in the temperature range -10 °C to 120 °C. The state of the sample can be monitored online using in situ UV-Vis spectroscopy to identify radiation damage, and measure precise sample concentration and aggregation state.

User Guide

Expertise:

Screening for X-ray diffraction of macromolecules

In-house data collection and processing

In-situ evaluation of sensitive crystallization targets, experimental phasing experiments – evaluation for MIR, SAD and MAD approaches

SAXS data measurement and evaluation

Synchrotron-based data collection and processing, structure solution

Instruct Centre

BIOCEV

BIOCEV

Prumyslova 595

25250, Vestec, Prague

Czech Republic

www.biocev.eu

X-ray Diffraction and Bio-SAXS, Vestec near Prague, Czech Republic

Contacts:

Jan Stránský
Jan Stránský
Institute of Biotechnology ASCR
Login to contact
Jiri Pavlicek
Jiri Pavlicek
BIOCEV - Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec
Login to contact