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About Macromolecular Crystallisation, Amsterdam, Netherlands

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The Protein Facility of the Netherlands Cancer Institute (NKI) in Amsterdam has a strong record in structural biology using macromolecular X-ray crystallography, Electron Microscopy and sample preparation, and is also extremely well-equipped for studying molecular biophysics.

We are very experienced in macromolecular crystallization and offer to our users:

- Robotics for preparation of nanoliter crystallization drops in 96-well format.

- Storage of crystallization plates at 4°C or 20°C.

- Screening and ranking of crystallization conditions in 96-well format (TOPAZ, AutoInspex station).

Solving crystal structures is beyond the scope of the facility!

 

Instruments Available:

The Facility has access to a high-throughput macromolecular crystallisation platform. Liquid handling robots are available for preparation of nanoliter crystallisation drops in 96-well format, including a 96-well pipetting robot (CyBi-SELMA) for filling up reservoir wells and a Mosquito for creating nanoliter crystallisation drops. Crystallisation trials can be stored and imaged by two RockImager systems, one at 4 °C and one at 20 °C. When suitable crystals are obtained for x-ray diffraction analysis, the applicant has to collaborate with a crystallography facility. Solving crystal structures is beyond the scope of the facility.

Instruct Centre

Protein facility - NKI

NKI Protein Facility

Plesmanlaan 121

1066CX

Amsterdam

Netherlands

www.nki.nl/topmenu/protein-facility/

Macromolecular Crystallisation, Amsterdam, Netherlands

Contacts:

Anastassis Perrakis
Anastassis Perrakis
The Netherlands Cancer Institute
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Patrick Celie
Patrick Celie
The Netherlands Cancer Institute
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Hans Wienk
Hans Wienk
The Netherlands Cancer Institute
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