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About Solution NMR, University of Leeds, UK

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About solution NMR, Leeds, UK

The NMR facility of the Astbury BioStructure Laboratory in Leeds has several unique capabilities available at 950 MHz. The first is based on direct heteronuclear detection (15N and 13C, 5mm TXO Cryoprobe). The combination of high field and Carbon detection has advantages for complex or large intrinsically disordered proteins. There are also new developments in characterising protein sidechains utilising Carbon detection. Techniques using 15N detection are under development and are of interest in chemically exchanging systems, as an alternative to Ca detection and when deuteration is not feasible. The second is based on using a smaller diameter probe (3mm TCI Cryoprobe) for mass limited samples and high ionic strength samples for optimised sensitivity.

Instruments Available:

Solution NMR 950 MHz Bruker Avance IIIHD (22.3 T)

950 MHz Ascend Aeon Bruker magnet with Avance IIIHD console with 4 channels and dual receivers; Automatic sample changer for up to 24 NMR tubes; Cryo TXO, (15N, 13C, 1H, 2H) 5mm, z-gradients, cold amplifiers on 15N, 13C, 1H and 2H, for 15N and 13C direct detection experiments; Cryo TCI (1H, 13C, 15N, 2H), 3mm, z-gradients, cold amplifiers on 13C, 1H and 2H.

Instruct Centre

Astbury Biostructure Laboratory

Astbury Centre for Structural Molecular Biology

University of Leeds

Leeds

United Kingdom

Solution NMR, University of Leeds, UK

Contacts:

Rebecca Thompson
Rebecca Thompson
University of Leeds
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Arnout Kalverda
Arnout Kalverda
University of Leeds
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