Service / Technology Instance

About Protein Production in E. coli with Isotope Labelling for NMR, Grenoble, France

View All Protein Production at Instruct

The isotope labelling platform of the Institut de Biologie Structurale (IBS) is devoted to the large scale production of proteins uniformly or specifically enriched in stable isotopes (15N, 13C and 2H) for biomolecular NMR spectroscopy studies. The platform offers access to innovative labelling schemes, developed locally on the platform by the IBS. The platform benefits from extensive scientific contacts with the IBS high field NMR Spectroscopy and Cell-Free platforms. Activities proposed by the platform: Users have access to the facility for large scale expression of labelled proteins in E.coli, under the supervision of a qualified platform engineer. Dedicated bench, optimised protocols and adequate isotopically labelled materials are available to users.

Instruments Available:

The Isotopic Labelling Platform of the IBS is devoted to feasibility studies or large-scale production of perdeuterated proteins enriched with stable isotopes (15N, 13C, 2H) or specifically methyl labelled for NMR spectroscopy studies. The platform is offering access to innovative labelling schemes, developed locally on the platform by the IBS NMR group. The platform benefits from extensive scientific contacts with the IBS high field NMR Spectroscopy and Cell-Free platforms. The isotopic labelling Platform is setting up a quality process aimed at an ISO 9001 labelling.

User Guide

Access

All required adaptations, scale-up and productions of labelled materials are performed on the platform by the user under supervision of a qualified engineer. Until summer 2013, due to lack of space, the platform can only satisfy a limited number of projects/year. Priority will be given to the project requiring advanced isotopic labelling schemes developed locally.

Cost For Users

Academics : The cost of isotopic materials and consumables used for the production of proteins will be refunded by the users to the IBS platform.

Approved Instruct Users : Access costs (Consumables, travel and accommodation) can be covered by Instruct.

Private companies : quotation under request.

How to make a request

A request form should be sent to the platform by email. Following receipt of the form, we will study the feasibility of your request within 1 month. If the request is accepted, we will contact you in order to propose a date for the access to the platform. Alternatively if a request cannot be satisfied, external users can have access to our protocols in order to express the protein in their lab.

Clic here to download the request form, and explain briefly your project, the staff in charge of the platform will contact you.

Instruct Centre

Instruct Centre FR2

ISBG & IBS

71 avenue des Martyrs

Grenoble

France

www.isbg.fr

Protein Production in E. coli with Isotope Labelling for NMR, Grenoble, France

Contacts:

Jerome Boisbouvier
Jerome Boisbouvier
CNRS
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Isabel Ayala
Isabel Ayala
CNRS
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