Service / Technology Instance

About Protein Production in E. coli with Isotope Labelling for NMR, CERM/CIRMMP, Florence, Italy

View All Protein Production at Instruct

The isotope labelling platform at CERM/CIRMMP is devoted to the large scale production of proteins uniformly enriched in stable isotopes (15N, 13C and 2H) for biomolecular NMR spectroscopy studies. The platform is offering protein expression in bacterial cultures grown on chemically defined minimal media, as well as advanced protein purification approaches with the unique possibility of running them in anaerobicity. Users have access to the facility for large scale expression of labelled proteins in E. coli, under the supervision of a qualified platform engineer. Dedicated bench, optimised protocols and adequate isotopically labelled materials are available to users.

Instruments Available:

The isotope labelling platform installed at CERM/CIRMMP offers the possibility of large scale production of a 15N- and/or 15N,13C-labelled protein with labeling methodologies ranging from the standard uniform labeling to the most recent advanced selective labeling schemes. The platform benefit of the interaction with a number of scientists from different scientific areas.

The isotope labelling platform installed at CERM/CIRMMP offers the possibility of large scale production of  a 15N,13C-,2H-labelled protein with a number of labeling methods ranging from the standard uniform labeling to the selective labeling schemes. The platform benefit of the interaction with a number of scientists from different scientific areas.

User Guide

CERM/CIRMMP isotope labelling platform combines a large set of expression vectors for Escherichia coli and protocols, which enable a broad screening of soluble protein expression in labeled media through the use of multiple fusion proteins and various growth conditions.

CERM/CIRMMP isotope labelling expression platform offers two modes of access

  1. The platform provides the expression vectors to the external users who perform cloning at their home institutions and come to CERM/CIRMMP to perform the expression assays in minimal media.
  2. The external users perform cloning and expression assays in minimal media at the CERM/CIRMMP platform.

Support includes training and supervision, as well as help with data analysis which is especially crucial for complex systems. The facility has a long-term expertise in producing metallo-proteins.

Cost For Users

Approved Instruct Users: Access costs (Consumables, travel and accommodation) can be partially or fully covered by Instruct, depending on the general costs.

Academics: The cost of isotopic materials and consumables used for the production of proteins will be refunded by the users to the CERM/CIRMMP platform.

Private companies: quotation under request.

How to make a request

A request form should be sent to the administrators of the platform by email. Following receipt of the form, we will study the feasibility of your request within one month. If the request is accepted, we will contact you in order to propose a date for the access to the platform. Alternatively if a request cannot be satisfied, external users can have access to our protocols in order to express the protein in their lab

Instruct Centre

Instruct Centre IT

CERM/CIRMMP

Via Sacconi 6

50019 Sesto

Florence

Italy

www.cerm.unifi.it

Protein Production in E. coli with Isotope Labelling for NMR, CERM/CIRMMP, Florence, Italy

Contacts:

Simone Ciofi-Baffoni
Simone Ciofi-Baffoni
CERM/CIRMMP
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Francesca Morelli
Francesca Morelli
CERM/CIRMMP
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