Analytical ultracentrifugation (AUC) is a method for characterisation of macromolecules and their complexes. It can be applied to homogeneous and heterogeneous mixtures of proteins, carbohydrates and nucleic acids. For systems with well-defined stoichiometries it can provide weight values for individual species and sedimentation coefficient values, which combined provide a measure of shape. The Oxford centre has two AUCs: an XL-A providing absorbance optical detection and an XL-I providing absorbance and interference optical detection. The samples can vary from ~2kDa up to 100MDa and the absorbance (wavelengths 190-800nm) and interference means that optical properties of different molecules can be harnessed to their specific detection.
Microcal iTC200 Microcalorimeter
Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. UNcle combines 3 different measurement modes — fluorescence, SLS and DLS. So you can crank out all your data in just a few hours, and use way less protein. All the info you’ll get makes picking the best formulation or protein construct a piece of cake.