EMBL Grenoble offers access to automated nano-volume crystallization screening and optimization services. Additional services include sample quality assessment and buffer optimization through TSA and estimation of crystallization likelihood. This platform is remotely operated through the web via the Crystallographic Information Management System (CRIMS) providing real-time access to results and experimental parameters.
Mail-in, fully automated, remote controlled protein-to-structure pipeline integrating crystallization screening and optimization automated crystal harvesting and cryocooling and automated or remote X-ray data collection. Based on CrystalDirect technology the CRIMS software.
The HTX lab offers automated high throughput crystallization screening and crystal processing including automated soaking, harvesting and cryocooling based on the CrystalDirect technology.
EMBL Grenoble operates the High Throughput Crystallization Facility (HTX lab), a large scale user facility offering HT crystallography services. Since the start of operations in 2003 the HTX lab has provided services to over 800 scientists and processes more than 1000 samples per year. The HTX lab has a strong focus in the development of new methods in macromolecular crystallography, including methods for sample evaluation and quality control ( Dupeux et al., 2011. Acta Cryst. D67,Mariaule et al., 2014Methods in Molecular Biology1091) and the CrystalDirect technology, enabling fully automated crystal mounting and processing (Cipriani & Marquez 2012 Acta Cryst. D68, 1393–1399; Zander et al. (2016) Acta Cryst. D72, 454-466). The HTX lab has also developed the Crystallographic Information Management System (CRIMS), a web-based laboratory information system that provides automated communication between crystallization and synchrotron data collection facilities, enabling uninterrupted information flow over the whole sample cycle from pure protein to diffraction data. This software is currently in operation in multiple laboratories across Europe. Through the combination of the CrystalDirect technology and the CRIMS software the HTX lab has developed the concept of Online Crystallography: Fully automated, remote controlled crystallography pipelines integrating crystallization screening, crystal optimization, crystal mounting and cryo-cooling and automated X-ray data collection (at ESRF, PETRA III and SLS synchrotrons) into continuous workflows controlled though dedicated web interfaces. This approach minimizes the delay between crystal growth and measurement, accelerating the progression of very challenging projects. The CrystalDirect technology also enables automated crystal soaking making it possible to support automated, large scale small molecule and Fragment Screening through crystallography efficiently and in a short time.
Currently we offer access to the following pipelines: