The Protein Facility of the Netherlands Cancer Institute (NKI) in Amsterdam has a strong record in structural biology using macromolecular X-ray crystallography, Electron Microscopy and sample preparation, and is also extremely well-equipped for studying molecular biophysics.
We are very experienced in macromolecular crystallization and offer to our users:
- Robotics for preparation of nanoliter crystallization drops in 96-well format.
- Storage of crystallization plates at 4°C or 20°C.
- Screening and ranking of crystallization conditions in 96-well format (TOPAZ, AutoInspex station).
Solving crystal structures is beyond the scope of the facility!
The Facility has access to a high-throughput macromolecular crystallisation platform. Liquid handling robots are available for preparation of nanoliter crystallisation drops in 96-well format, including a 96-well pipetting robot (CyBi-SELMA) for filling up reservoir wells and a Mosquito for creating nanoliter crystallisation drops. Crystallisation trials can be stored and imaged by two RockImager systems, one at 4 °C and one at 20 °C. When suitable crystals are obtained for x-ray diffraction analysis, the applicant has to collaborate with a crystallography facility. Solving crystal structures is beyond the scope of the facility.