Service / Technology Instance

About Electron Microscopy, University of Leeds, UK

The Astbury Biostructure Laboratory includes the electron microscopy facility within the Faculty of Biological Sciences. We have a range of state-of-the-art equipment for transmission electron microscopy of biological specimens, from small macromolecular complexes to cells, tissues and organisms.

Our facilities are available to researchers from across the University of Leeds, and external users form academia and industry.

Our facility can operate at Biosafety Level II.

Instruments Available:

If you already have grids prepared, you should apply for Titan Krios access for high resolution data acquisition (single particle or electron tomography). If you require assistance during project work up, for example optimisation by negative stain, making/optimising/screening cryo-EM grids, please apply for enhanced support, where we can assign the appropriate microscopes for the project stage. 

Optimised for single particle projects. Falcon III direct electron detector, EPU.   

Optimised for electron tomography and single particle of small/flexible macromolecular complexes. Falcon II direct electron detector, Gatan energy filtered BioQuantum967, volta-potential phase plate. Software includes EPU, Tomography 4 and GMS 3.

User Guide

We have two state-of-the-art Titan Krios microscopes equipped with direct electron detectors (DED) for high resolution imaging. Krios 1 is equipped with a FEI Falcon 3 DED and is primarily used for single particle data collection (using FEI EPU software) of macromolecular complexes. Krios 2 is equipped with an energy filtered Gatan K2 summit and a phase plate therefore this microscope is ideal for tomography data collection (Tomo software) and single particle of macromolecular complexes (EPU software).

If you already have grids prepared, you can apply for Titan Krios access for high resolution data acquisition (single particle or electron tomography). If you require assistance during project work up, for example optimisation by negative stain, making/optimising/screening cryo-EM grids, please apply for enhanced support, where we can assign the appropriate microscopes for the project stage. 

Instruct Centre

Astbury Biostructure Laboratory

Astbury Centre for Structural Molecular Biology

University of Leeds

Leeds

United Kingdom

Electron Microscopy, University of Leeds, UK

Contacts:

Rebecca Thompson
Rebecca Thompson
University of Leeds
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Cryo EM team at Leeds admin account
Cryo EM team at Leeds admin account
University of Leeds
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