Service / Technology Type

Surface Plasmon Resonance (SPR)

Highly sensitive analysis of binding phenomena in real-time without the need for labeling or size limitations

Instruct has 0 centres offering Surface Plasmon Resonance (SPR) across Europe. Navigate the map and click on the pins to discover centres near you.

Details

In cellular structural biology the analysis of biomolecular interactions is crucial for describing interaction networks and monitoring multiple complex formation. Surface Plasmon Resonance (SPR) allows a highly sensitive analysis of binding phenomena in real-time without the need for labeling or size limitations. SPR can be used for determining equilibrium constants (Kd), stoichiometry, and association (ka) and dissociation (kd) rates in weak and strong interacting systems, and has applications in molecular assembly, epitope mapping, and small molecule screening. In SPR experiments, one of the interacting moieties is immobilized onto the biosensor surface and the other is free in the solution that is made to flow over the biosensor surface. The response in SPR signal is proportional to the mass of the material bound to the biosensor surface, so large interacting molecules give a more intense signal than small ones. The availability of specific sensor chips for different application purposes makes SPR a powerful tool in structural biology and a basic resource for Instruct.

Service/Technology Instances

Surface Plasmon Resonance  (SPR)

Surface Plasmon Resonance (SPR) Experts

Nicole Thielens
Nicole Thielens
Institut de Biologie Structurale
Login to contact expert
Terms of Use | Terms of Submission | Privacy Policy | Copyright © 2018 The University of Oxford | Powered by ARIA