Joint Instruct-ARBRE MOBIEU Workshop: Analysis and optimisation of sample quality for cryo-electron microscopy and other structural techniques
The Joint Instruct-ARBRE MOBIEU Workshop, held in Brno, Czech Republic, in February 2020 was attended by structural biologists looking to improve their skills in sample analysis and optimisation for structural techniques, in particular Cryo-EM. Training highlighted the importance of basic protein quality control as well as buffer and storage optimisation, using quick and relatively cheap techniques, to save precious Cryo-EM time. The workshop combined practical and theoretical sessions to provide the trainees an understanding of the relationship between sample quality and structural technique output, as well as practical knowledge of sample preparation.
The training school school was organised by Instruct-ULTRA and ARBRE-MOBIEU (COST Action CA15126).
The lectures given during this event can be found below.
Importance of sample quality for downstream experimental studies
Duration: 25 minutes Presenter: Arthur Sedivy
Expression systems for production of protein complexes
Duration: 30 minutes Presenter: Peggy Stolt-Bergner
Methods to determine sample homogeneity
Duration: 27 minutes Presenter: Alexander Fish
Sample optimisation for single particle cryoEM with ProteoPlex and Massphotometry
Duration: 27 minutes Presenter: David Haselbach
Analysis of protein folding by CD spectroscopy
Duration: 28 minutes Presenter: Arthur Sedivy
Sample quality studied by mass spectrometry
Duration: 24 minutes Presenter: Ondrei Sedo
Analytical ultracentrifugation as a technique to assess the sample quality
Duration: 25 minutes Presenter: Jan Komarek
Cryo-EM techniques overview
Duration: 32 minutes Presenter: Jiri Novacek
Optimising the sample preparation for Cryo-EM studies
Duration: 55 minutes Presenter: Holger Stark