3rd Instruct-ERIC Best Practices in CryoEM Workshop
The 3rd Annual Best Practices in CryoEM Workshop was held in November 2019 at CBI/IGMBC in Strasbourg, France. The workshop provided the opportunity for EM facility scientists, managers and computational experts in academia and industry to gather and exchange knowledge and experience. The talks given at the event can be found below.
Welcome and Introduction: Future National Centre for Biomedical CryoEM
Speaker: Bruno Klaholtz
Duration: 22 minutes
The cryoWriter: Modular microfluidics for protein isolation, EM-grid preparation, and “differential visual proteomics"
Speaker: Luca Rima
Duration: 40 minutes
Cryo electron tomography workflows at NeCEN
Speaker: Rebecca Dillard
Duration: 34 minutes
In Situ Structural Biology - You bring the samples, we the tools!
Speaker: Philipp Erdmann
Duration: 29 minutes
In situ structure determination using Cryo-FIB-SEM, CryoET and subtomogram averaging
Speaker: James Gilchrist
Duration: 27 minutes
Data acquisition for high-resolution subtomogram averaging: the details
Speaker: Wim Hagen
Duration: 34 minutes
Some aspects of running a large CryoEM Facility: user access and data collection strategies
Speaker: Shaoxia Chen
Duration: 40 minutes
On-the-fly tilt series processing in Warp
Speaker: Dmitry Tegunov
Duration: 24 minutes
Scipion 2.0 for facilities
Speaker: Pablo Conesa
Duration: 37 minutes
Opportunities and challenges in cryoEM sample preparation
Speaker: Rebecca Thompson
Duration: 20 minutes