Provides Israel’s premier facilities in structure determination techniques
Protein production in bacteria/baculovirus/mammalian cells. Proteins are typically purified following a standard 3 step protocol which includes: affinity capture, ion exchange and size exclusion. Specific techniques can be employed depending on the protein, the experimental requirements, tags and yields. Removal of tags with TEV protease can be employed.
CryoSTEM and CryoTEM tomography on a Tecnai F20 with Quantum/K2 camera and BF/DF/HAADF STEM detectors
Also room temperature STEM tomography of thick plastic sections