Instruct Centre IL - ISPC (WIS) Israel


Provides Israel’s premier facilities in structure determination techniques


234 Herzl Street

7610001

Rehovot

Israel

ISPC (WIS) Israel website

Flagship Service/Technology at ISPC (WIS) Israel:

Protein production in bacteria/baculovirus/mammalian cells. Proteins are typically purified following a standard 3 step protocol which includes: affinity capture, ion exchange and size exclusion. Specific techniques can be employed depending on the protein, the experimental requirements, tags and yields. Removal of tags with TEV protease can be employed.

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Other Services/Technologies at ISPC (WIS) Israel:

CryoSTEM and CryoTEM tomography on a Tecnai F20 with Quantum/K2 camera and BF/DF/HAADF STEM detectors

Also room temperature STEM tomography of thick plastic sections



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Crystallization screening: Utilizing the sitting and hanging drop by vapor diffusion methods for crystallizing soluble proteins, using the Mosquito, and LCP Mosquito crystallization robots.



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Scientific Contacts
Gideon Schreiber
Gideon Schreiber

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Joel Sussman
Joel Sussman

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Admin Contacts
Shira Albeck
Shira Albeck

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Natasha Gruber
Natasha Gruber

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