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About X-ray Diffraction and Bio-SAXS, Brno, Czech Republic

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The X-ray Diffraction and Biological Small-Angle X-Ray Scattering (Bio-SAXS) core facility is equipped with top-class instruments for diffraction experiments with single crystal samples focused on the determination of the 3-D structure of (macro)molecules down to atomic resolution and for small angle X-ray scattering experiments with isotropically scattering samples focused on determination of the shape and size of macromolecules or nanoparticles. 

Instruments Available:

Rigaku BioSAXS-1000 SAXS camera for small angle X-ray scattering from solutions of biological macromolecules.

Rigaku HighFlux HomeLab™ robotised macromolecular diffraction system with ACTOR sample changer optimised for work at Cu-Kα wavelength.Rigaku HighFlux HomeLab™ universal, dual wavelength (Mo-Kα and Cu-Kα) diffractometer.

User Guide

The range of applicable molecular mass for diffraction methods: from 100 up to 1,000,000, where the lower value covers molecules significant for nanotechnology, materials science or pharmacology and the upper limit covers bio-macromolecules such as nucleic acids, proteins and their complexes. 

Range of applicable particle sizes for SAXS: from 2 to 100 nm.

Unique Features

The diffraction of X-rays in single crystal samples is the most important and – if an appropriate sample is available – also the fastest methodology currently available for the determination of the atomic structures of molecules and/or macromolecules and their complexes.

On the other hand, SAXS is a technique capable of determining structural characteristics such as mono dispersity or aggregation, oligomeric state, low resolution three-dimensional shape or even quaternary structure not from a crystal but from a solution of (bio) macromolecules.

The centralised organisation of expensive instrumentation for SAXS and single crystal studies and highly trained staff allow the cost-effective use of resources and obtaining of experimental results in rapid responses to the demands of even untrained users.

Instruct Centre

CEITEC

CEITEC

Kamenice 753/5

625 00

Brno

Czech Republic

www.ceitec.eu

X-ray Diffraction and Bio-SAXS, Brno, Czech Republic

Contacts:

Tomas Klumpler
Tomas Klumpler
CEITEC - Central European Institute of Technology
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Jaromir Marek
Jaromir Marek
CEITEC - Central European Institute of Technology
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Katerina Vagnerova
Katerina Vagnerova
CEITEC - Central European Institute of Technology
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Radka Matejickova
Radka Matejickova
CEITEC - Central European Institute of Technology
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