The Bijvoet Centre houses solid state 950, 800, 700, 500 and 400 MHz NMR equipment for the characterisation of biomolecules, their structure determination and dynamical and functional studies. All of the NMR instruments are state-of-the-art digital Bruker NMR spectrometers.
Not only are the standard pulse sequences for spectroscopic, structural, dynamical, and functional characterisation available, the Bijvoet Centre also offers tailored pulse sequences for structural determination of high molecular weight proteins and other special applications such as in-cell NMR experiments. Dynamic Nuclear Polarization (DNP) capabilities on the 800 and 400 MHz instruments provide a significant boost in sensitivity.
The Bijvoet Center has solid state 950, 800, 700, 500 and 400 MHz NMR equipment for the characterization of biomolecules, their structure determination and dynamical and functional studies. All of the NMR instruments are state-of-the-art, digital Bruker NMR spectrometers. In addition to all the standard pulse sequences for spectroscopic, structural, dynamical, and functional characterization, tailored pulse sequences for structural determination of high molecular weight proteins and other special applications such in-cell NMR experiments are also available. Dynamic Nuclear Polarization (DNP) capabilities on the 800 and 400 MHz instruments provide a signficant boost in sensitivity.
For more information, please visit the website of the SONNMRLSF facility at the Bijvoet Centre.
For iNEXT-Discovery applications:
Scientific background, significance and objectives
Please describe the general scientific background for your project, explain - to a non-expert in the biology of the system - why these questions are important, and clearly at the end define your objectives, in terms of the tangible results you want to obtain and the questions these results will help to address.
Project background in your lab & recent results
Please describe previous work in the lab, leading to this request. Please clarify the features of your material for this characterization (e.g. protein crystals, biomaterials, fibrils, lyophilized material etc.) and comment on the sample purity, amount and stability. If you are planning to use experiments that need labeling, please clarify if these are available, or how you plan to make them.
Research required, requested
Please list the NMR experiments you are suggesting, and the conditions you plan to use (i.e. MAS up to … and temperature down to …). For each experiment, please clarify if you have the necessary equipment (e.g. rotors, caps, sealing - some redundancy with above is acceptable). If you need to prepare the samples while on site (e.g. spin-labelling, rotor packing, …) please indicate that. If you are unsure of the optimal method to address your question, please state this openly and use this space to explain as well as possible what are the exact questions that you need to answer; this will not affect your proposal negatively as part of our mission is to suggest the best experiment(s) for answering user questions.
Benefit for health, food, biotechnology or biomaterials
Please include a statement for the immediate or longer-term impact that your research might have in innovations related to the fields above. These do not need to lead to a “product” but to scientific or technical innovations that can be of value for translational research in any of the sectors above.