Structural mass spectrometry (MS3D) offers various methodologies for characterisation of protein structure. The Institute of Biotechnology/Centre of Molecular Structure (IBT/CMS) at BIOCEV offers different labelling approaches including hydrogen/deuterium exchange, covalent labelling, chemical cross-linking and limited proteolysis. The facility is equipped with cutting-edge technologies including high-resolution mass spectrometer, HPLC system, H/D system and in-house software for data processing.
The service provided includes data processing and reporting ready for publication. The platform also offers: identification and quantification of proteins, precise determination of protein molecular mass, and characterisation of various post translational modifications.
Ultra high performance liquid chromatography system for separation of proteins, peptides or metabolites connected to the 15T-FTICR mass spectrometer.
Bruker Daltonics 15T-Solarix XR FT-ICR mass spectrometer, with electrospray and MALDI ion sources, and with Agilent Technologies 1200 HPLC system (usually coupled to the 15T-SolariX XR mass spectrometer) for the separation of complex peptide mixtures, proteins and metabolites.
Simple MALDI-TOF/TOF mass spectrometer for routine analysis of proteins and peptides.
Excimer laser dedicated mostly for FPOP experiments.
Separation of protein mixtures
Intact protein analysis
Native mass spectrometry
Characterisation of protein modifications
Protein surface covalent labelling
Hydrogen/deuterium exchange (HDX)
Data processing and interpretation of mass spectrometric data