SPR is a technique for measuring the binding between interactants (e.g. proteins) in real time. Usually one protein is covalently linked on a sensor chip, while a second protein (or any other molecule) is made to flow across the surface of the chip.
SPR can be used to investigate several biomolecular interactions:
SPR analysis can provide answers to several questions:
SPR analysis can be performed at a wide range of buffers, pH, and temperatures.
A few micrograms of ligand (the immobilized partner) per chip surface are required. Samples and buffers must be filtered through a 0.2 micron filter, and de-gassed before use.
The measurement consists of the following steps:
All of the components required for interaction analysis are available at the facility:
Different immobilization strategies can be used:
SPR has a wide range of applicability:
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