Recombinant expression of proteins and their complexes using the baculovirus/insect cell expression system (BEVS)
The baculovirus expression vector system (BEVS) is a powerful eukaryotic method to produce proteins and protein complexes. BEVS uses a recombinant baculovirus carrying the heterologous genes of interest, to infect insect cell cultures provided as monolayers or suspension. BEVS has become particularly attractive for protein research as it combines the ability to produce difficult-to-express, eukaryotic proteins with high yields and often authentic processing (PTMs) with simple cultivation needs without particular safety precautions. Important technological advances have greatly improved upon the original procedures for the generation of recombinant baculoviruses which were time consuming and required specialised expertise. Streamlined and cost-effective operation procedures for baculovirus-based expression have become available, and the BEVS is now accessible also to non-specialist users. Within the Infrastructure, new technologies have been developed and implemented which enable efficient and rapid production of large multiprotein complexes with many subunits, in the quality and quantity required for high-resolution structural and functional studies.
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