Robotein offers automated biophysical protein analysis, together with screening for optimal cloning and expression, and protein production
Robotein is a versatile technological platform for high-throughput (HT) protein production and analysis. It is built on competences and infrastructures available in the academic setting of two labs that offer a complete structural biology portfolio: the Centre for Protein Engineering at the Université de Liège and the Structural Biology and Bioinformatics Centre at the Université Libre de Bruxelles. We perform automated screening for cloning, gene expression, purification and biophysical analysis on either a collaborative or service basis. The HT screening approach serves to optimize the labour-intensive downstream steps, i.e. largescale production and detailed biochemical and biophysical studies.
Robotein is equipped with two robotic workstations of the Microlab STAR line by Hamilton, two microplate readers (Infinite M200 PRO by Tecan) allowing UV/Vis absorbance and fluorescence measurements, together with chemiluminescence detection, a system (LabChip GXII, Caliper Life Sciences by Perkin Elmer) for automated electrophoretic separation of proteins and nucleic acids, an Octet HTX platform (fortéBio – Pall Life Sciences) allowing high throughput (HT) analysis of biomolecular interactions and quantitation of biomolecules even in crude mixtures (e.g. cell lysates, hybridoma supernatants), in 96- and 384- well microplates. Furthermore, the combination of a protein spotter (Arrayjet Marathon Classic Microarrayer) with an infrared imager (Agilent Cary 620 FTIR imaging microscope) allows fast and reliable quantification of many protein samples, together with determination of protein secondary structure content and measurement of phosphorylations and glycosylations.
Our services include: